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ti2 inverted widefield microscope  (Nikon)


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    Nikon ti2 inverted widefield microscope
    Ti2 Inverted Widefield Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59766 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ti2+inverted+microscope/Objectives/pmc12989073-235-10-9
    Average 99 stars, based on 59766 article reviews
    ti2 inverted widefield microscope - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    other:

    Article Title: The phase-separating Magnaporthe oryzae MoSpa2 complex organizes actin nucleation centers for plant infection.
    Article Snippet: All rights reserved.. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints.. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.

    Article Title: A critical role for the Fascin family of actin bundling proteins in axon development, brain wiring and function.
    Article Snippet: All labeled coverslips were mounted on glass slides using Fluoromount-G (Southern Biotech #0100–01) for imaging.

    Imaging:

    Article Title: Morphodynamics of human early brain organoid development.
    Article Snippet: .. Imaging was performed on a Nikon Ti2 inverted microscope, coupled to a Crest X-Light V3, equipped with Teledyne Kinetix back-thinned cameras. .. The objective used was a Nikon Apochromat 40×/1.15 water immersion LWD (MRD77410) with water supply at 60 μl h−1 for 25 h. The following imaging conditions were used: DAPI at 80% power (100 ms), GFP at 80% power (300 ms), RFP at 80% power (300 ms) and Cy5 at 80% power (300 ms).

    Article Title: Focused ultrasound-mediated APOE4 knockdown in mouse brain.
    Article Snippet: .. Imaging was performed using Nikon Ti2 inverted microscope with AXR resonant spectral scanning confocal unit under 20× and 60× magnification. ..

    Inverted Microscopy:

    Article Title: Morphodynamics of human early brain organoid development.
    Article Snippet: .. Imaging was performed on a Nikon Ti2 inverted microscope, coupled to a Crest X-Light V3, equipped with Teledyne Kinetix back-thinned cameras. .. The objective used was a Nikon Apochromat 40×/1.15 water immersion LWD (MRD77410) with water supply at 60 μl h−1 for 25 h. The following imaging conditions were used: DAPI at 80% power (100 ms), GFP at 80% power (300 ms), RFP at 80% power (300 ms) and Cy5 at 80% power (300 ms).

    Article Title: Focused ultrasound-mediated APOE4 knockdown in mouse brain.
    Article Snippet: .. Imaging was performed using Nikon Ti2 inverted microscope with AXR resonant spectral scanning confocal unit under 20× and 60× magnification. ..

    Article Title: Fabrication of cell culture hydrogels by robotic liquid handling automation for high-throughput drug testing.
    Article Snippet: .. One image per well was captured using a Ti2 inverted microscope (Nikon) equipped with an air 20× objective (NA 0.75, MRD00205). .. Lumencor CELESTA Light Engine with 546 nm (laser power 16 ± 2 mW, exposure 50 ms), 477 nm (28 ± 3 mW, 50 ms), 446 nm (20 ± 2 mW, 10 ms), and 638 nm (37 ± 4 mW, 200 ms) wavelengths was used to image cell actin, tubulin, and nuclei in G1 and S/G2/M, respectively.

    Article Title: Fabrication of cell culture hydrogels by robotic liquid handling automation for high-throughput drug testing.
    Article Snippet: .. A Ti2 inverted microscope (Nikon) was equipped with a 40× silicon oil objective (NA 1.25) (MRD73400, Nikon). .. Lumencor CELESTA Light Engine with 546 nm (16 ± 2 mW, 200 ms), 477 nm (28 ± 3 mW, 30 ms), 446 nm (20 ± 2 mW, 10 ms), and 638 nm (37 ± 4 mW, 60 AR TI CL E IN P RE SS ms) wavelengths was used to image cell actin, tubulin, and nuclei in G1 and S/G2/M, respectively.

    Article Title: Fabrication of cell culture hydrogels by robotic liquid handling automation for high-throughput drug testing
    Article Snippet: .. One image per well was captured using a Ti2 inverted microscope (Nikon) equipped with an air 20× objective (NA 0.75, MRD00205). .. Lumencor CELESTA Light Engine with 546 nm (laser power 16 ± 2 mW, exposure 50 ms), 477 nm (28 ± 3 mW, 50 ms), 446 nm (20 ± 2 mW, 10 ms), and 638 nm (37 ± 4 mW, 200 ms) wavelengths was used to image cell actin, tubulin, and nuclei in G1 and S/G2/M, respectively.

    Article Title: Fabrication of cell culture hydrogels by robotic liquid handling automation for high-throughput drug testing
    Article Snippet: .. A Ti2 inverted microscope (Nikon) was equipped with a 40× silicon oil objective (NA 1.25) (MRD73400, Nikon). .. Lumencor CELESTA Light Engine with 546 nm (16 ± 2 mW, 200 ms), 477 nm (28 ± 3 mW, 30 ms), 446 nm (20 ± 2 mW, 10 ms), and 638 nm (37 ± 4 mW, 60 ms) wavelengths was used to image cell actin, tubulin, and nuclei in G1 and S/G2/M, respectively.



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    Image Search Results


    Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions

    doi: 10.1016/j.mtbio.2026.103015

    Figure Lengend Snippet: Synthesis and characterization of BA-HPCS@CGRP microspheres based on microfluidic fabrication. A. Fourier transform infrared spectroscopy spectra of the HPCS, 3-Carboxyphenylboronic acid (BA), and BA-HPCS. B. The hydrogel precursors appear as a liquid macroscopically before gelation. C. The hydrogels appear milky white after photo-crosslinking. D. The imaging of BA-HPCS@CGRP microspheres based on microfluidic chips: macroscopic and microscopic observations. E. Particle size distribution of BA-HPCS@CGRP microspheres. F and G. Representative scanning electron microscope images of BA-HPCS@CGRP microspheres. H. The pore size distribution of lyophilized BA-HPCS@CGRP microspheres. I. The releasing of CGRP from BA-HPCS@CGRP in PBS and different glucose conditions (100 mg/dL, 400 mg/dL). J. Representative live/dead fluorescence images of L929 cells after co-culture with microspheres (green calcein-AM for live cells, red propidium iodide for dead cells). K. The quantitative analysis of L929 cell viability co-cultured with microspheres. ns, no significance. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Following the incubation, fluorescence images were captured using a Nikon inverted fluorescence microscope (Nikon, Japan, Modle: Eclipse Ti2-E).

    Techniques: Fourier Transform Infrared Spectroscopy, Spectroscopy, Imaging, Microscopy, Pore Size, Fluorescence, Co-Culture Assay, Cell Culture